www.伊人-www.一区二区三区.com-www.一区-www.一级片.com-青草视频网址-青草视频网站在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>HTB-103KATO III細(xì)胞, 人胃癌細(xì)胞

KATO III細(xì)胞, 人胃癌細(xì)胞

簡(jiǎn)要描述:KATO III細(xì)胞, 人胃癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):HTB-103
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問(wèn)  量:1769

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

KATO III細(xì)胞, 人胃癌細(xì)胞

OrganismHomo sapiens, human
Tissue

stomach:derived from metastatic pleural effusion; supraclavicular and axillary lymph nodes and Douglas cul-de-sac

Product Formatfrozen
Morphologyspherical
Culture Propertiesmixed, adherent and suspension
Biosafety Level1
Diseasegastric carcinoma
Age55 years adult
Gendermale
EthnicityAsian
Storage Conditionsliquid nitrogen vapor phase
KaryotypeThe stemline chromosome number is hypotetraploid with the 2S component occurring at 6.2%. Nine markers were common to most S metaphases, four markers were less frequent. One (occasionally 2 copies) homogenous staining region (HSR) (t(11;HSR) was present in all metaphases examined, but no double minutes (DM) were detected.
Clinical Data

55 years adult

Asian

male

TumorigenicYes
Effects

Yes, in cheek pouches of anti thymocyte serum treated hamsters

No, in nude mice

Complete Growth MediumThe base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
KATO III細(xì)胞, 人胃癌細(xì)胞
Subculturing

Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove culture medium with floating cells to a centrifuge tube. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. 2.. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 3. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 4. To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximay 125 xg for 5 to10 minutes. 5. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. 6. Place culture vessels in incubator at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: 5% CO2 in air recommended

Temperature: 37°C          






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 91大神在线精品网址| 91桃色在线看片| 99在线观看精品视频| bbw丰满大肥奶肥婆| 边摸边吃奶边做动态图邪我粉33| 91久久亚洲最新一本| 中国在线观看www视频| 一二三四视频中文字幕在线看| gaysex中国小伙| 中国同性男男gayxxxx免费的| 女人扣逼| a国产精品| 色狠狠一区二区三区香蕉蜜桃| 欧美老幼欢| 91精品视频网站| 在线a人片天堂免费观看高清| 久久熟| chinese 男同 野外 gay| 肉动漫在线免费观看| 奇米第四狠狠777高清秒播| 乱人伦中文字幕在线不卡网站| 2021国产在线视频| 老师您的兔子好软水好多图片| 巨胸美女喂我吃她的乳| 99精品视频99| np高h肉辣一女多男| 欧美xxxx69tube8护士| 麻豆va一区二区三区久久浪| 欧美肥肥婆另类xxxx000| 日韩孕交japanese孕交| oh我的鬼神大人韩剧网在线观看| 中国一级毛片aaa片| 北条麻妃大战黑人hd| 奇米影视777欧美在线观看| 日韩伦乱| 玖玖精品视频| 久草免费福利在线| chinese基友gay勾老师| 99精品视频观看| 日本私人噜噜影院| 被公侵犯的岬奈奈美88av|