www.伊人-www.一区二区三区.com-www.一区-www.一级片.com-青草视频网址-青草视频网站在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>HTB-37人結(jié)腸腺癌細(xì)胞

人結(jié)腸腺癌細(xì)胞

簡(jiǎn)要描述:HTB-37 Caco-2 人結(jié)腸腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):HTB-37
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問(wèn)  量:1893

產(chǎn)品分類

Product Category

詳細(xì)介紹

HTB-37 Caco-2 人結(jié)腸腺癌細(xì)胞

ATCC® Number: HTB-37™

Designations: Caco-2

Depositors: J Fogh

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

HTB-37 Caco-2 人結(jié)腸腺癌細(xì)胞

Source: Organ: colon

Disease: colorectal adenocarcinoma

Cellular Products: keratin

retinoic acid binding protein 1

retinol binding protein 2

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.

Applications: transfection host (Nucleofection technology from Lonza

Roche FuGENE® Transfection Reagents)

Receptors: heat stable enterotoxin (Sta, E. coli), expressed

epidermal growth factor (EGF), expressed

Virus Susceptibility: Human immunodeficiency virus 1

Tumorigenic: Yes

Reverse Transcript: N

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 11,13,14

D16S539: 12,13

D5S818: 12,13

D7S820: 11,12

TH01: 6

TPOX: 9,11

vWA: 16,18

Cytogenetic Analysis: The stemline modal chromosome number is 96, occurring at 16% with polyploidy at 3.2%. Ten common markers were detected i.e., t(1q;?), 10q-, t(11q17q) and 7 others. The t(1q17q) and M11 were found in a portion of cells. The ins(2), 10q-, and t(15q;?) were generally paired, and t(11q;17q) and t(21q;?) were mostly three-copied. Normal N9 was absent, and N21 was lost in some cells. One to 4 small acrocentric chromosomes were detected. No Y chromosome with bright distal q-band was detected by Q-observation.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

Me-2, 1

PGM1, 1

PGM3, 1

Age: 72 years adult

Gender: male

Ethnicity: Caucasian

HeLa Markers: N

Comments: Upon reaching confluence, the cells express characteristics of enterocytic differentiation [PubMed ID: 1939345]. Caco-2 cells express retinoic acid binding protein I and retinol binding protein II [PubMed ID: 9040537].

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.The recommended inoculum is 1 X 10(4) viable cells/cm2. Subculture cells when they are about 80% confluent, at a cell concentration between 8 X 10(4) and 1 X 10(5) cell/cm2.

Incubate cultures at 37C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:6 is recommended

Medium Renewal: 1 to 2 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Doubling Time: about 62 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

derivative:ATCC CRL-2102

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

References: 18385: Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708

22409: Jumarie C, Malo C. Caco-2 cells cultured in serum-free medium as a model for the study of enterocytic differentiation in vitro. J. Cell. Physiol. 149: 24-33, 1991. PubMed: 1939345

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

22564: Adachi A, et al. Productive, persistent infection of human colorectal cell lines with human immunodeficiency virus. J. Virol. 61: 209-213, 1987. PubMed: 3640832

22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

23009: Cohen MB, et al. Receptors for Escherichia coli heat stable enterotoxin in human intestine and in a human intestinal cell line (Caco-2). J. Cell. Physiol. 156: 138-144, 1993. PubMed: 8100232

HTB-37 Caco-2



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 成人免费在线观看视频| 久久人人爽人人爽a大片| 模特三级在线观看| 可以免费看黄的网址| 免费视频网站在线看视频| 91高清免费视频| freexxx性欧美孕交孕妇| 99av在线| 青青视频免费观看在线播放| 2021一卡2卡三卡4卡乱码不卡| 欧美日韩亚洲国产精品一区二区| 91精品视频网站| 日韩综合在线观看| 一级一黄在线观看视频免费| 男女性生夜晚视频| 色老头xxxwww作爱视频| 操人视频在线观看| 久久精品免费观看| 嫩草影院在线观看精品| 动漫痴汉电车1~6集在线| 北岛玲heyzo在线播放| 九九久久精品视频| 日本视频免费播放| 女人的逼毛片| 91po在线观看国产永久地址| 大尺度np女主有奶| 中文字幕一区久久久久| 欧美三级在线| 成人 在线播放| av福利| a毛片在线看片免费| freehd另类xxxx| 日本淫| 丁香花在线高清免费观看| 琼明神女录在线| 青草国产在线| 最新精品在线视频| 久久久www免费看片| 91精品国产闺蜜国产在线| 欧美乱大交xxxxx另类| 大黑人暴力性猛交|